Functional RNA polymerase II promoters in solitary retroviral long terminal repeats (LTR-IS elements).
نویسندگان
چکیده
LTR-IS elements are middle repetitive sequences in the mouse genome with structural features of solitary retroviral LTRs. In order to get some insight in the possible functional role of these sequences the promotor activity of two LTR-IS representatives differing by 105 bp in their U3 region was investigated. Gene fusions between LTR-IS sequences and the bacterial gene coding for chloramphenicol acetyl transferase (CAT) were transfected into mouse 3T6 cells and the expression of CAT was measured. It is shown that the LTR-IS sequences represent weak RNA polymerase II promoters which require enhancement by cis-or trans-activating factors.
منابع مشابه
Family of middle repetitive DNA sequences in the mouse genome with structural features of solitary retroviral long terminal repeats.
Screening of a 129/J mouse genomic library under nonstringent hybridization conditions with a xenotropic virus-like long terminal repeat (LTR) probe revealed a family of sequences resembling insertion elements (IS) with structural features of solitary retroviral LTRs; these are called LTR-IS. They are interspersed among variable flanking regions of mouse DNA and lack any viral structural genes....
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 13 7 شماره
صفحات -
تاریخ انتشار 1985